Well, like the title says, nothing works! I feel like I've been busting my butt trying to get experiments to work. I'm trying to characterize a totally uncharacterized protein picked up in a loss of function screen with a super cool (if not so penetrant) phenotype. I made an antibody. And I'm trying (desperately) to figure out if it works, and where my protein is expressed and localized. Nothing works. Or it half works, or it just doesn't make sense and things are conflicting. Or the controls don't even work in the experiment. *sigh* So much for my group meeting/progress report this week. Summary: no progress made, oh except for the realization that I screwed up the cloning of my overexpression contructs and had to remake everything (at least I think I'm mostly caught back up with completed constructs). Not the best way to end my nearly 5 year stay in the lab (it's likely to be my last presentation before I leave in September). I am bummed. Why won't SOMETHING, ANYTHING work?